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flow cytometric analysis  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec flow cytometric analysis
    Flow Cytometric Analysis, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 2025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometric+analysis/MACSQuant+Analyzer+10/pmc13236643-57-0-12
    Average 98 stars, based on 2025 article reviews
    flow cytometric analysis - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Software:

    Article Title: Validation of LILR antibody specificities and development of LILRA3-specific antibodies
    Article Snippet: Subsequent washes were performed using the same washing buffer, after which a propidium iodide (PI, Dojindo) solution at a concentration of 0.4 μg/mL was added to the wells. .. Flow cytometric analysis was conducted using the MACSQuant Analyzer 10 (Miltenyi Biotec), and data were analyzed using FlowJo software (BD Biosciences). ..

    Article Title: MUC1-targeted CAR-T cell secreted anti-PD-1 IgG antibody enhances antitumor activity in Cholangiocarcinoma.
    Article Snippet: To assess CAR transduction efficiency, cells were stained with Alexa Fluor® 488-conjugated goat anti-human IgG (H+L) antibody (cat: 109-605-003; Jackson ImmunoResearch) for 15 minutes at 4°C to detect the human IgG CH3 domain in the CAR spacer region as previously described [24]. .. Flow cytometric analysis was conducted using the MACSQuant® Analyzer 10 (Miltenyi Biotec), and data were processed and analyzed using FlowJo software, version 10.7.1 (BD Biosciences). .. Enzyme-linked immunosorbent assay (ELISA) AR TIC LE IN PR ES S To evaluate the binding of secreted anti-PD-1 Ig, culture supernatant from CAR-T cells was collected on day 4 following transduction and stored at -20°C.

    Article Title: Glycan-based biological degraders targeting the cytokine immune axis
    Article Snippet: .. Flow cytometric analysis was performed on a MACSQuant® Analyzer 10 (Miltenyi Biotec), and data were analyzed using FlowJo software (version 10.1). ..

    Article Title: Glycan-based biological degraders targeting the cytokine immune axis.
    Article Snippet: .. Flow cytometric analysis was performed on a MACSQuant® Analyzer 10 AR TI CL E IN P RE SS (Miltenyi Biotec), and data were analyzed using FlowJo software (version 10.1). ..

    Article Title: A clinical-grade automated platform for the manufacturing of CAR-γδ T cells for immunotherapy
    Article Snippet: In all cases 7-AAD staining solution (Miltenyi Biotec) was used to assess cell viability and exclude dead cells. .. Flow cytometric analysis was carried out on a MACSQuant ® Analyzer 10 (Miltenyi Biotec) using MACSQuantify Software version 2.13. ..

    Marker:

    Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT-Immortalised Mesenchymal Stromal Cells.
    Article Snippet: .. BM-MSC/TERT292 EVs enriched in different collection media were characterised in terms of surface marker expression by Multiplex bead-based EV analysis followed by a flow cytometric analysis (MACSPlex EV Kit MSC, human130-136-864, Miltenyi Biotec). ..

    Expressing:

    Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT-Immortalised Mesenchymal Stromal Cells.
    Article Snippet: .. BM-MSC/TERT292 EVs enriched in different collection media were characterised in terms of surface marker expression by Multiplex bead-based EV analysis followed by a flow cytometric analysis (MACSPlex EV Kit MSC, human130-136-864, Miltenyi Biotec). ..

    Multiplex Assay:

    Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT-Immortalised Mesenchymal Stromal Cells.
    Article Snippet: .. BM-MSC/TERT292 EVs enriched in different collection media were characterised in terms of surface marker expression by Multiplex bead-based EV analysis followed by a flow cytometric analysis (MACSPlex EV Kit MSC, human130-136-864, Miltenyi Biotec). ..

    Article Title: Immortalization of mesenchymal stromal cells by hTERT does not affect the functional properties of secreted extracellular vesicles.
    Article Snippet: .. Surface protein profiling of primary and TERT EVs was performed via Multiplex bead-based EV analysis followed by a flow cytometric analysis (MACSPlex EV IO Kit, human, 130-108-813, Miltenyi Biotec). ..

    Flow Cytometry:

    Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia
    Article Snippet: .. Cells were collected at 48 h post-electroporation to assess the percentage of live and eGFP positive cells via flow cytometric analysis using a MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec). .. Briefly, cells were lifted with Accutase (ThermoFisher Scientific, cat. no. A1110501), quenched with PBS (ThermoFisher Scientific, cat. no. BP3991) supplemented with 2% FBS (PBS+FBS; Peak Serum, cat. no. PSFB4) and spun at 300 x g for 5 min.



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    Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow cytometric quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Biomimetic liposomes synergize with high-intensity focused ultrasound to induce ferroptosis in tumors

    doi: 10.1016/j.apsb.2026.02.024

    Figure Lengend Snippet: Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow cytometric quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.

    Article Snippet: Subsequently, flow cytometric analysis was performed on the cells using FlowJo v.7.6.5 (Tree Star, Inc., Ashland, USA).

    Techniques: Liposomes, Zeta Potential Analyzer, Cryo-EM Sample Prep, CCK-8 Assay, Flow Cytometry, Labeling, Fluorescence, Confocal Microscopy, Expressing, Comparison